plasmid mruby2 n1 Search Results


mruby2 n1  (Addgene inc)
93
Addgene inc mruby2 n1
Mruby2 N1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mruby2 n1/product/Addgene inc
Average 93 stars, based on 1 article reviews
mruby2 n1 - by Bioz Stars, 2026-05
93/100 stars
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mruby2  (Addgene inc)
94
Addgene inc mruby2
Figure 4. Microtubules Confine SPK1 Signaling Nodules to the Branch Apex (A) YFP:SPK1 punctae are clustered within a microtubule-depletion zone. Right: representative intensity plot of YFP:SPK1 and <t>mRUBY2:MBD</t> signal along the cell periphery below the yellow line. (B) Microtubule organization in developing tri- chomes expressing GFP:MBD with and without oryzalin treatment. Microtubules are completely depolymerized after 1.5 hr of oryzalin treatment, but recovered after inhibitor washout. (C–E) SPK1 localization becomes progressively unfocused following oryzalin treatment, and the effect is reversible following inhibitor washout (C). Perimeter length of the SPK1 signal, normalized by tip radius of curvature, as a function of oryzalin treatment time and inhibitor washout (D). Signal intensity of the cortical SPK1 signal normalized to local cytosolic signal intensity as a function of oryzalin treatment time and inhibitor washout (E). Mean ± SD (n = 22). *p < 0.05, **p < 0.01 (ANOVA with Tukey HSD test). Scale bars represent 5 mm. See also Figure S4.
Mruby2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mruby2/product/Addgene inc
Average 94 stars, based on 1 article reviews
mruby2 - by Bioz Stars, 2026-05
94/100 stars
  Buy from Supplier
mruby2-n1 3xflag (plasmid #118980)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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mruby2-nt-n1 (plasmid #54612)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


Figure 4. Microtubules Confine SPK1 Signaling Nodules to the Branch Apex (A) YFP:SPK1 punctae are clustered within a microtubule-depletion zone. Right: representative intensity plot of YFP:SPK1 and mRUBY2:MBD signal along the cell periphery below the yellow line. (B) Microtubule organization in developing tri- chomes expressing GFP:MBD with and without oryzalin treatment. Microtubules are completely depolymerized after 1.5 hr of oryzalin treatment, but recovered after inhibitor washout. (C–E) SPK1 localization becomes progressively unfocused following oryzalin treatment, and the effect is reversible following inhibitor washout (C). Perimeter length of the SPK1 signal, normalized by tip radius of curvature, as a function of oryzalin treatment time and inhibitor washout (D). Signal intensity of the cortical SPK1 signal normalized to local cytosolic signal intensity as a function of oryzalin treatment time and inhibitor washout (E). Mean ± SD (n = 22). *p < 0.05, **p < 0.01 (ANOVA with Tukey HSD test). Scale bars represent 5 mm. See also Figure S4.

Journal: Current biology : CB

Article Title: Microtubule-Dependent Confinement of a Cell Signaling and Actin Polymerization Control Module Regulates Polarized Cell Growth.

doi: 10.1016/j.cub.2018.05.076

Figure Lengend Snippet: Figure 4. Microtubules Confine SPK1 Signaling Nodules to the Branch Apex (A) YFP:SPK1 punctae are clustered within a microtubule-depletion zone. Right: representative intensity plot of YFP:SPK1 and mRUBY2:MBD signal along the cell periphery below the yellow line. (B) Microtubule organization in developing tri- chomes expressing GFP:MBD with and without oryzalin treatment. Microtubules are completely depolymerized after 1.5 hr of oryzalin treatment, but recovered after inhibitor washout. (C–E) SPK1 localization becomes progressively unfocused following oryzalin treatment, and the effect is reversible following inhibitor washout (C). Perimeter length of the SPK1 signal, normalized by tip radius of curvature, as a function of oryzalin treatment time and inhibitor washout (D). Signal intensity of the cortical SPK1 signal normalized to local cytosolic signal intensity as a function of oryzalin treatment time and inhibitor washout (E). Mean ± SD (n = 22). *p < 0.05, **p < 0.01 (ANOVA with Tukey HSD test). Scale bars represent 5 mm. See also Figure S4.

Article Snippet: The MYB5 promoter, mRUBY2 and MBD-nos terminator were amplified from pEGADMYB5pro (provided by D Marks, University of Minnesota, St. Paul, MN), pFA6a-link-yomRuby2-Kan (Addgene, Cambridge, MA) and GFP:MBD [36], respectively.

Techniques: Expressing